Analysis and interpretation of enzyme kinetic data

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Analysis and interpretation of enzyme kinetic data

[email protected] Abstract Analysis of enzyme kinetic data to obtain valid information requires attention to two details that are often given less attention than they need. The first is an experimental design that ensures that the variables treated as independent are truly independent, that different interpretations can be distinguished, and that parameter values can be estimated. The second is ...

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The concerted two-state allosteric model proposed by Monod et al. (Monod, J., Wyman, J., and Changeux, J.-P. (1965) J. Mol. Biol. 12, 88-118) is extended to expressions representing the initial velocity as a function of substrate concentration for two-substrate enzymes for which random substrate binding and rapid equilibration of all binding steps prior to a rate-determining conversion in the t...

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Many organophosphorus compounds are irreversible inhibitors of acetylcholinesterase. The methods used in the literature to determine the inhibition kinetic constants usually involve either manual determination of the slope at various points along the inhibition progress curve or fitting polynomials to the curve. The present study investigates the use of non-linear-regression analysis to determi...

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Most biological processes require an enzyme to act as a catalyst in order for the reaction to take place. With the exception of catalytic RNA, all enzymes are proteins. Most importantly, enzymes are highly effective in catalyzing diverse chemical reactions in a regulated, selective, and specific manner. With a few exceptions, most reactions catalyzed by enzymes can be described satisfactorily b...

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ژورنال

عنوان ژورنال: Perspectives in Science

سال: 2014

ISSN: 2213-0209

DOI: 10.1016/j.pisc.2014.02.010